C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
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چکیده
منابع مشابه
A Novel Vector for Expression/Secretion of Properly Folded Eukaryotic Proteins: a Comparative Study on Cytoplasmic and Periplasmic Expression of Human Epidermal Growth Factor in E. coli
Expression of eukaryotic proteins in E. coli often results in their aggregation. Proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. This is not achieved in cytoplasmic expression in E. coli because of the absence of disulfide bonds formation. A novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...
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Existing conditional expression systems can be classified in two major categories that are based either on the induction or on the de-repression of transcription. The system described here combines both mechanisms, since a unique transcription factor can be shifted from a repression to a stimulation activity by simply changing its ligand. The resulting advantage of this system is the complete a...
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Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verifica...
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15 صفحه اولa novel vector for expression/secretion of properly folded eukaryotic proteins: a comparative study on cytoplasmic and periplasmic expression of human epidermal growth factor in e. coli
expression of eukaryotic proteins in e. coli often results in their aggregation. proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. this is not achieved in cytoplasmic expression in e. coli because of the absence of disulfide bonds formation. a novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2019
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkz069